Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7

CyQuant® GR Dye : One 25 µL tube 4 Cotton Swabs (Part No described in Assay Protocol References 1 Ridley

Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7 This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

CyQuant® GR Dye : One 25 µL tube 4 Cotton Swabs (Part No described in Assay Protocol References 1 Ridley

cyquant protocol A two-step warming protocol was shown to improve the cell recovery in this adherent cell model Frozen plates were removed from -135°C storage and placed

patented CyQuant® GR Dye Assay Principle Page 3 3 Related picture) and quantified by CyQuant® GR Dye as described in the Assay Protocol

cyquant protocol Cyquant) • Remove plate from the machine and place it on ice • Wash 2Â protocol • Defrost assay plate and add 200 μL of lysis buffer per well

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